Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe

Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe

Blog Article

Jun Zhou*, Yi-Ting Tsai*, Hong Weng, Ewin N Tang, Ashwin Nair, Digant P Davé, Liping Tang, Department of Bioengineering, University of Texas at Arlington, Arlington, TX *Both authors contributed equally to this workAbstract: Neutrophils play an important role in implant-mediated inflammation and infection.Unfortunately, current methods which monitor neutrophil activity, including enzyme measurements and histological evaluation, Advanced Wound Care - Cleansers require many animals and cannot be used to accurately depict the dynamic cellular responses.To understand the neutrophil interactions around implant-mediated inflammation and infection it is critical to develop methods which can monitor in vivo cellular activity in real time.In this study, formyl peptide receptor (FPR)-targeting near-infrared nanoprobes were fabricated.

This was accomplished by conjugating near-infrared dye with specific peptides having a high affinity to the FPRs present on activated neutrophils.The ability of FPR-targeting nanoprobes to detect and quantify activated neutrophils was assessed both in vitro and in vivo.As expected, FPR-targeting nanoprobes preferentially accumulated on activated neutrophils in vitro.Following transplantation, FPR-targeting nanoprobes preferentially accumulated at the biomaterial implantation site.

Equally important, a strong relationship was observed between the extent of fluorescence intensity Tech in vivo and the number of recruited neutrophils at the implantation site.Furthermore, FPR-targeting nanoprobes may be used to detect and quantify the number of neutrophils responding to a catheter-associated infection.The results show that FPR-targeting nanoprobes may serve as a powerful tool to monitor and measure the extent of neutrophil responses to biomaterial implants in vivo.Keywords: in vivo imaging, nanoprobe, neutrophils, inflammation, biocompatibility.